Titlebook: Diabetic Nephropathy; Methods and Protocol Luigi Gnudi,David A. Long Book 2020 Springer Science+Business Media, LLC, part of Springer Natur

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Sample Preparation and Stereological Methods for the Study of Glomerular Ultrastructure Using ElectrIn this chapter we describe conventional methods used for preparing renal tissue for transmission electron microscopy. We also describe a relatively new technique, serial block face scanning electron microscopy. Protocols are given for processing, sectioning, and imaging of tissue along with methods for obtaining quantitative data from the results.

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Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathyhapter describes a method of isolating and culturing podocyte cells from mouse adult kidneys. In this way, podocytes with genetic modifications can be obtained from transgenic animals and they can be used to study the effects of the diabetic environment in vitro.

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Legitimation durch Kommunikation?es known as slit diaphragms that contribute to the glomerular filtration barrier. The disruption of podocyte biology or slit diaphragm formation in humans is a precursor to albuminuria, renal failure, and cardiovascular morbidity. Due to genetic and functional similarities, the nephrocytes of . are

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Regierungssysteme und Demokratietypen progressive disease or test novel therapeutics. In this chapter we describe in detail the experimental procedures required to conduct the . rodent model, in which hyperglycemia and renin-dependent hypertension synergize to promote moderate proteinuria, renal fibrosis, and induction of many of the t

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Regierungssysteme und Demokratietypenhapter describes a method of isolating and culturing podocyte cells from mouse adult kidneys. In this way, podocytes with genetic modifications can be obtained from transgenic animals and they can be used to study the effects of the diabetic environment in vitro.

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https://doi.org/10.1007/978-3-663-01446-1ease. Glomerular lesions constitute the most striking and consistent features identified in biopsies from patients with DKD, although tubulointerstitial injury has an important and often under-recognized role in the progression to overt nephropathy. In advanced stages of the disease, podocyte detach