Titlebook: Developmental Toxicology; Methods and Protocol Craig Harris,Jason M. Hansen Book 2012 Springer Science+Business Media, LLC 2012 antioxidant

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Gene Knockdown by Morpholino-Modified Oligonucleotides in the Zebrafish (,) Model: Applications forcular and mechanistic level while establishing a relationship between these molecular events and morphological, behavioral, and/or physiological effects (i.e., phenotypic anchoring)..In this chapter, we address important considerations for using MOs to study developmental toxicology in zebrafish embryos and provide a protocol for their use.

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Avian Models in Teratology and Developmental Toxicology,e direct study of an agent’s embryotoxic effects. Here, we present protocols for using avian embryos in toxicology research, including egg husbandry and hatch, toxicant delivery, and assessment of proliferation, apoptosis, and cardiac structure and function.

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The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test System,during embryogenesis and the mechanisms by which chemicals interfere with critical signaling pathways. In this system, uniform developmental stages are selected for assessment, exposures are controlled precisely, and the confounding influences of maternal metabolism and transport are avoided.

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Analysis of Nrf2-Mediated Transcriptional Induction of Antioxidant Response in Early Embryos,hods used to analyze Nrf2-mediated transcriptional induction of antioxidant response in mouse embryos. Data which have been generated by using these methods have also been included to provide examples of their usefulness in evaluating Nrf2-mediated antioxidant response in early embryos.

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