Titlebook: Developmental Biology Protocols; Volume III Rocky S. Tuan,Cecilia W. Lo Book 2000 Humana Press 2000

Incorporate

collagen

注入

Application of Antisense Oligodeoxynucleotides in Developing Chick Embryoss a particularly useful tool to study the function of a gene in a specific target tissue, as it acts in a spatially (i.e., site of injection) and temporally (i.e., time of treatment) restricted manner.

Decimate

Relative Reverse Transcription-Polymerase Chain Reactionotopes. Northern blot analysis requires at least ten times as much sample, radioisotopes, and the results obtained are, at best, semiquantitative. Northern blot analysis also is technically more difficult since electrophoresis of mRNA, a blotting step, and autoradiography are required.

FLINT

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确定无疑

有常识

Methods for Double Detection of Gene Expressioncombined whole mount . hybridization and β-galactosidase staining allows for simultaneous detection of mRNA and transgene-directed β-galactosidase expression. Simultaneous detection allows for the most direct comparison of expression patterns. These procedures are derived from protocols used in . (.) and mice (.,.).

FLUSH

https://doi.org/10.1007/978-1-4471-6799-0 study of development, a panoramic collection of experimental models of morphogenesis, development, and cellular differentiation are detailed. Both in vivo and in vitro systems are included. The volume concludes with various examples of developmental models of diseases and their molecular basis.

BILL

Guide to Teaching Computer Sciences a particularly useful tool to study the function of a gene in a specific target tissue, as it acts in a spatially (i.e., site of injection) and temporally (i.e., time of treatment) restricted manner.