Titlebook: Detection of Mitochondrial Diseases; Frank Norbert Gellerich,Stephan Zierz Book 1997 Springer Science+Business Media Dordrecht 1997 genome

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Non-invasive quantitative ,P MRS assay of mitochondrial function in skeletal muscle ,e resolution . NMR measurements of phosphocreatine, inorganic phosphate and ATP resonances were conducted on human forearm flexor muscle during involuntary twitch contraction at eight different frequencies. Mitochondrial and glyco(geno)lytic ATP synthesis fluxes, and the cytosolic free energy of ATP

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Phosphorus metabolite distribution in skeletal muscle: Quantitative bioenergetics using creatine anaact cell through the advent of .P-NMR spectroscopy. The near-equilibrium formulation for creatine kinase (CK) has been used for the calculation of ADP. and ATP chemical potential in many of these studies. However, control of the bulk cytoplasmic PCr/Cr ratio by the ATP/ADP ratio through CK implies t

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OTTER

Fluxes through cytosolic and mitochondrial creatine kinase, measured by P-31 NMR kinase (CK) iso-enzyme family has a multi-faceted role in cellular energy metabolism and is characterized by a complex pattern of tissue-specific expression and subcellular distribution. In mammalian tissues, there is always co-expression of at least two different CK isoforms. As a result, previous

OTTER

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Small scale preparation of skeletal muscle mitochondria, criteria of integrity, and assays with refereparation was developed for 25–100 mg tissue with pigeon breast muscle as model organ. The yield was 40%. Data collected during the developmental work were used to evaluate criteria of mitochondrial quality. The cytochrome c conservation, i.e. cytochrome c per mitochondrial quantity in the preparat

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