Titlebook: Dendritic Cells; Methods and Protocol Vanja Sisirak Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to S

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https://doi.org/10.1007/978-3-030-31368-5 a more rigorous and global assessment. Here, we describe a cost-effective and robust in vitro differentiation system for the production of cDC1s and pDCs equivalent to their blood counterparts, from cord blood CD34. hematopoietic stem cells (HSCs) cultured on a stromal feeder layer with a combinati

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Using AutoML for AI Service Deploymente-derived DCs, Axl-DCs, and several other subsets. Here, we review the specific phenotypes, functions, and localization within the tumor microenvironment (TME) of human DC subsets thanks to flow cytometry and immunofluorescence but also with the help of high-output technologies such as single-cell RNA sequencing and imaging mass cytometry (IMC).

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https://doi.org/10.1007/978-1-4614-3058-2dendritic cells (DCs) and CD4. T cells using genetically engineered LIPSTIC mice. This protocol requires expertise in animal experimentation and multicolor flow cytometry. Once mouse crossing has been achieved, it takes 3 days or more to complete, depending on the kinetics of the interactions that the researcher wishes to investigate.

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Monitoring the Interaction Between Dendritic Cells and T Cells In Vivo with LIPSTICdendritic cells (DCs) and CD4. T cells using genetically engineered LIPSTIC mice. This protocol requires expertise in animal experimentation and multicolor flow cytometry. Once mouse crossing has been achieved, it takes 3 days or more to complete, depending on the kinetics of the interactions that the researcher wishes to investigate.