Titlebook: Dendritic Cells; Methods and Protocol Vanja Sisirak Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to S

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Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/265511.jpg

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https://doi.org/10.1007/978-1-0716-2938-3DCs; Ontogeny; Cell differentiation; Functional characterization; Immune system

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In Vitro Generation of Murine Dendritic Cells from Hoxb8-Immortalized Hematopoietic Progenitorst of ERHBD-Hoxb8-expressing progenitors with estrogen results in Hoxb8 activation, which blocks cell differentiation and allows for expansion of homogenous progenitor cell populations in the presence of FLT3L. These cells, referred to as Hoxb8-FL cells, retain lineage potential for lymphocyte and my

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In Vitro Generation of Murine CD8α+ DEC205+ XCR1+ Cross-Presenting Dendritic Cells from Bone Marrow–which mouse primary bone marrow cells are cocultured with OP9 stromal cells expressing Notch ligand Delta-like 1 (OP9-DL1) to produce CD8α. DEC205. XCR1. cDC1 (Notch cDC1). This novel method provides a valuable tool to facilitate the generation of unlimited cDC1 for functional studies and translatio

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Clonal Analysis of Human Dendritic Cell Progenitors Using a Stromal Cell Cultureentials of single human hematopoietic stem and progenitor cells (HSPCs) to multiple DC subsets, myeloid and lymphoid cells that will facilitate investigation of human DC lineage specification and reveal its molecular bases.

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Matthias E. Liechti,Friederike Holzet of ERHBD-Hoxb8-expressing progenitors with estrogen results in Hoxb8 activation, which blocks cell differentiation and allows for expansion of homogenous progenitor cell populations in the presence of FLT3L. These cells, referred to as Hoxb8-FL cells, retain lineage potential for lymphocyte and my

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