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Titlebook: Dendritic Cell Protocols; Stephen P. Robinson,Andrew J. Stagg,Stella C. Knig Book 20011st edition Humana Press 2001

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https://doi.org/10.1007/978-1-4842-3108-1erdigitating DC that stimulate T cells in the secondary lymphoid tissue (.). In the blood, two populations of DC can be identified based on the expression of the β-intergrin, CD1 1c. For most individuals, the proportion of DC that are CD1 1c. ranges from 30% to 70%. The CD11c. population was origina
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https://doi.org/10.1007/978-1-4842-3108-1cells (LC) by the presence of Birbeck granules. LC are considered to belong to the family of dendritic cells (DC) that are important for the initiation of immune responses (.). In the dermis, macrophages and DC are present (.,.). The expression of CD1a molecules can be used to identify DC in the ski
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https://doi.org/10.1007/978-1-4842-3108-1form a rare cell population in the lung and early studies were hampered because scarce cell populations are seldom easy to isolate. Besides, this cell is phenotypically heterogeneous depending on its localization, differentiation, or activation status. This chapter will elaborate on the heterogeneit
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Contact Problems and Their Solution,itic cells (DC) characterized from each compartment have different properties. The methods given are based on cell sorting for isolation of cells, and flow cytometry and immunohistochemical staining for analysis of cells. Myeloid non-T cells are first enriched by density gradient centrifugation, she
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Scabies and Secondary InfectionsThe method described in this chapter for the isolation of mouse thymic dendritic cells (DC) is an optimization of our previously published methods (.,.) and involves the following major steps:
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Isolation of Mouse Thymic Dendritic CellsThe method described in this chapter for the isolation of mouse thymic dendritic cells (DC) is an optimization of our previously published methods (.,.) and involves the following major steps:
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Murine Intestinal Dendritic Cell Isolationlarge intestine and from Peyer’s patches (PP). This technique can also be used to obtain intestinal macrophage-enriched populations. The characterization of an assay system, the allogeneic mixed leukocyte reaction (MLR), is also described.
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