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Titlebook: Dendritic Cell Protocols; Stephen P. Robinson,Andrew J. Stagg,Stella C. Knig Book 20011st edition Humana Press 2001

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发表于 2025-3-21 19:42:25 | 显示全部楼层 |阅读模式
书目名称Dendritic Cell Protocols
编辑Stephen P. Robinson,Andrew J. Stagg,Stella C. Knig
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Medicine
图书封面Titlebook: Dendritic Cell Protocols;  Stephen P. Robinson,Andrew J. Stagg,Stella C. Knig Book 20011st edition Humana Press 2001
描述Dendritic Cell Protocols provides chapter and verse for many useful practical approaches to the art of studying dendritic cells. The book gives information on the usual techniques for derivation of human dendritic cells from precursor stem cells, such as monocytes. In addition it provides data on the difficult tasks of isolating dendritic cells directly from different tissues; whether dendritic cells from precursor cells or from tissues of mouse or human are required, this book contributes practical information. The last section of the book is devoted to functional aspects of dendritic cells ranging from inf- mation relevant to cell migration to antigen uptake and T cell stimulation. But what is a dendritic cell? The explosion of studies on this cell type over the last few years is breathtaking. We now have so-called “myeloid” and “plas- cytoid” dendritic cells; are they of separate lineages or are they related? There are claims that dendritic cells can be derived from B cells and from granulocytes, as well as from “monocytes. ” Lymphocytes can be switched on or off by dendritic cells. Dendritic cells can preferentially cause Th1 or Th2 responses or some may stimulate B cells direc
出版日期Book 20011st edition
版次1
doihttps://doi.org/10.1385/1592591507
isbn_ebook978-1-59259-150-3Series ISSN 1543-1894 Series E-ISSN 1940-6037
issn_series 1543-1894
copyrightHumana Press 2001
The information of publication is updating

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发表于 2025-3-21 23:14:49 | 显示全部楼层
Isolation, Enrichment, and Culture of Murine Epidermal Langerhans Cells of methods for mass production of dendritic cells from blood or bone marrow) Langerhans cells served as a prototype dendritic cell. The basis of our current knowledge on the maturation of dendritic cells stems from experiments with epidermal Langerhans cells (.-.).
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https://doi.org/10.1007/978-1-4842-3108-1y that has to be taken into consideration when studying and isolating lung DC. Furthermore, techniques for the isolation of lung DC from human parenchymal lung tissue and bronchoalveolar lavage fluid (BAL) are described.
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https://doi.org/10.1007/978-3-319-02192-8 and granulocytes, or from myelomonocytic cells, which are also precursors of monocytes (.,.). DC can be propagated from progenitors in BM (.), blood (.), or secondary lymphoid tissues (.). In addition, peripheral blood mononuclear cells (PBMC) can develop into DC-like cells if cultured in the presence of GM-CSF and interleukin-4 (IL-4) (.,.).
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Contact Problems and Their Solution,ST is then given. This is based on the localization of the NFκB family member, RelB, to the nucleus of differentiated DC, and exclusion of B cells, macrophages, and follicular DC by double staining. Some variations that are particularly useful in frozen sections follow.
发表于 2025-3-23 09:36:47 | 显示全部楼层
Isolation and Propagation of Mouse Liver-Derived Dendritic Cells and granulocytes, or from myelomonocytic cells, which are also precursors of monocytes (.,.). DC can be propagated from progenitors in BM (.), blood (.), or secondary lymphoid tissues (.). In addition, peripheral blood mononuclear cells (PBMC) can develop into DC-like cells if cultured in the presence of GM-CSF and interleukin-4 (IL-4) (.,.).
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