Titlebook: Decoding the Antibody Repertoire; High Throughput Sequ Brandon DeKosky Book 2017 Springer International Publishing AG 2017 B Cell.Single-ce

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https://doi.org/10.1007/978-3-319-60961-4Obtaining an extensive sequence database for the naïve B cell repertoire is of high importance for immunology and medical research because the ensemble of sequences that comprise the naïve repertoire will ultimately dictate whether an organism has the ability to recognize a particular chemical species.

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Background,The last 30 years of the biotechnology revolution have to a great extent been fueled by the discovery and application of monoclonal antibodies for research and therapeutic purposes.

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In-Depth Determination and Analysis of the Human Paired Heavy and Light Chain Antibody Repertoire,The determination of immune receptor repertoires using high throughput (NextGen) DNA sequencing has rapidly become an indispensable tool for the understanding of adaptive immunity, antibody discovery and in clinical practice.

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,Paired VH:VL Analysis of Naïve B Cell Repertoires and Comparison to Antigen-Experienced B Cell RepeObtaining an extensive sequence database for the naïve B cell repertoire is of high importance for immunology and medical research because the ensemble of sequences that comprise the naïve repertoire will ultimately dictate whether an organism has the ability to recognize a particular chemical species.

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Bennett T. Amaechi BDS, MS, PhDbased technique with capacity for up to 10. cells per analysis [.], then translated the same general workflow into emulsion droplets for interrogation of up to 10. single B cells per operator in a single day.

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Book 2017have been applied for rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease..