Titlebook: DNA-Protein Interactions; Principles and Proto Benoît P. Leblanc,Sébastien Rodrigue Book 2015Latest edition Springer Science+Business Media

决定性

Georgios I. Doukidis,Ray J. Paulrder to regulate gene expression making it relevant to determine the profiles of cohabitation of several proteins on the chromatin fiber. Chromatin immunoprecipitation (ChIP) has been broadly used to determine the profile of several histone posttranslational modifications as well as transcription fa

impaction

Georgios I. Doukidis,Ray J. Pauluence-programmable tools for various purposes such as genome editing and transcriptional regulation. A critical aspect of the system is the selection and validation of spacer sequences that allow precise targeting of the guide RNA-Cas9 complex. We describe a procedure involving computational and exp

gonioscopy

EXALT

Studies in Computational Intelligencetranscriptional processes in that alteration of the interaction between its components results in the deregulation of cellular transcriptional program. Modification of epigenetic marks, variation in the precise positioning of nucleosomes, and consequent mobilization of nucleosomes regulate the acces

描绘

https://doi.org/10.1007/978-3-030-97269-1 it is often useful to represent the signal over known regions of interest, such as genes, using aggregate or individual profiles. In the present chapter, we describe and explain the best practices on how to generate such profiles as well as other usages of the versatile aggregate profiler (VAP) too

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Horizon

Electrophoretic Mobility Shift Assay Using Radiolabeled DNA Probes,col, a purified protein of interest is mixed with a 5′-end radiolabeled DNA probe. The bound complexes are separated by electrophoretic migration through a polyacrylamide gel and detected with a phosphorimager. The applications of EMSA are diverse, from thermodynamic and kinetic analyses to observat

新陈代谢

In Vitro DNase I Footprinting,using bulky nucleases such as DNase I. The DNase I footprinting method was developed to take advantage of this fact in the study of DNA-protein interactions: it consists in comparing the pattern of fragments generated by the partial digestion of a DNA sequence in the absence of a protein to that pro

CLAN

,Determining the Architecture of a Protein–DNA Complex by Combining FeBABE Cleavage Analyses, 3-D Prologous proteins from which the DNA binding can be inferred, and/or if only portions of the protein can be crystallized. If the protein comprises just a part of a large multi-subunit complex, other complications can arise such as the complex being too large for NMR studies, or it is not possible to

让步

In Cellulo DNA Analysis: LMPCR Footprinting,lation of gene expression. The nuclease-hypersensitive sites and sequences bound by transcription factors often correspond to genetic regulatory elements. Using the ligation-mediated polymerase chain reaction (LMPCR) technology, it is possible to precisely analyze these DNA sequences to demonstrate