Titlebook: DNA-Encoded Chemical Libraries; Methods and Protocol David Israel,Yun Ding Book 2022 Springer Science+Business Media, LLC, part of Springer

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Quantitation of DNA-Encoded Libraries by qPCR,eneral protocol using a double-stranded DNA binding dye for reaction monitoring. This allows the selection process to be assessed at each step prior to preparation for sequencing. The same method has additional applications in the practice of DEL technology.

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Target Protein Design and Preselection Analysis, the protein is of high quality, and that ligand binding sites are accessible under DEL selection conditions. Here we describe general considerations for target protein design and expression and experiments that are conducted before initiating selections to assess protein quality and validate methods that will be used for the DEL selection.

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On-DNA Pd and Cu-Promoted C-N Cross-Coupling Reactions, the chemical space which was assembled by the on-DNA chemical reactions. On-DNA C-N cross-coupling has great value for expanding the chemical space of DEL as amines are most abundant organic reagents available. Here we described the first on-DNA C-N cross-coupling reactions promoted by both Pd and Cu.

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DNA-Compatible Nitro Reduction and Synthesis of Benzimidazoles,l nitro groups to aryl amines by using sodium dithionite (Na.S.O.). The new protocol offers simple operation and circumvents the pyrophoric potential of the conventional method (Raney nickel). The utility of this method is demonstrated by the versatile synthesis of benzimidazoles on DNA.

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DNA Encoding of Natural Products, Herein, we describe a method for functionality-independent annotation of complex natural products with amplifiable DNA barcodes to generate a DNA-encoded natural product library (.DEL). This method provides a simple and practical solution to leverage natural products by DNA barcoding.

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One Bead-One Compound (OBOC) Peptidomimetic-Encoded Library Synthesis via Split-and-Pool Methods,be a simple and general method for the submonomer solid phase synthesis of large one bead-one compound (OBOC) peptidomimetic libraries of structurally diverse compounds that can be encoded by mass or genetic methods.

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Artificial Intelligencein Educationpargyl and 5′-azido oligonucleotides. We also present methods for the synthesis of phosphorothioate junctions from 3′-thiophospho and 5′-iodo oligonucleotides and for the synthesis of phosphodiester junctions from both 3′-hydroxy and 5′-phospho- and 3′-phospho and 5′-hydroxy oligonucleotides using 1-cyanoimidazole.

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