Titlebook: DNA and RNA Profiling in Human Blood; Methods and Protocol Peter Bugert Book 2009 Humana Press 2009 Blood cell antigens.Disease markers.Gen

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Book 2009Profiling in Human Blood: Methods and Protocols., leading international experts contribute both established and recently developed protocols for complex and high-throughput DNA and RNA profiling. Divided into two thorough sections, the volume concentrates on DNA profiling for blood cell antigens thr

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Real-Time PCR Assays for High-Throughput Blood Group Genotypingalidation of rapid and effective molecular methods. The genetic basis of the main alleles of the most important blood groups are known, but the frequencies vary in the different populations, thus for the genetic techniques to be efficient it is important to evaluate them, in order to adapt the molecular approaches.

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Limited Computation, Unlimited Designse blood is provided for potential transfusion recipients. The potential to dramatically lower the incidence of alloimmunization and to avoid serious hemolytic complications of transfusions can be realized with the implementation of this technology.

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Representational Flexibility for Designenerated a series of analytical tools to address the manufacturing, detection and data analysis components of a microarray experiment. In particular, we set up a universal array approach in combination with a PCR-LDR (polymerase chain reaction-ligation detection reaction) strategy for allele identification in the HLA gene.

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A. Giretti,L. Spalazzi,M. Lemma is designed to isolate platelets from 20 ml of peripheral blood. This chapter provides detailed protocols for microarray and SAGE transcript profiling. We also discuss peculiarities of platelet purification, RNA isolation, and transcript profiling.

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PCR–ELISA for High-Throughput Blood Group Genotypingevant minor blood group antigens were adapted to this method and are described in this work: Rh (D, C, c, E, e), Kell (K, k), Duffy (Fy., Fy.), and Kidd (Jk., Jk.). Other blood group antigens could be easily tested this way as long as their molecular basis is well established.

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Single Base Extension in Multiplex Blood Group Genotypingse blood is provided for potential transfusion recipients. The potential to dramatically lower the incidence of alloimmunization and to avoid serious hemolytic complications of transfusions can be realized with the implementation of this technology.

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Multiplex ,-Typing by Pyrosequencing proper matching of organ donor to recipient, the monitoring of . associated genetic risk to autoimmune diseases, population genetic studies, as well as evaluation of the genetics of human host–human pathogen interaction.