Titlebook: DNA and RNA Profiling in Human Blood; Methods and Protocol Peter Bugert Book 2009 Humana Press 2009 Blood cell antigens.Disease markers.Gen

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RNA Stabilization of Peripheral Blood and Profiling by Bead Chip Analysisemic diseases as well as the development of biomarkers for drug development. Since most cellular components of peripheral blood are specialized to quickly respond to exogenous stimuli, sample procurement approaches are required that reduce the overall impact of ex vivo changes in gene expression due

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Transcript Profiling of Human Platelets Using Microarray and Serial Analysis of Gene Expression (SAGnscripts. Taken together, these mRNAs constitute a platelet transcriptome. Platelets have a unique and reproducible transcript profile, which includes ∼1,600–3,000 individual transcripts. In this chapter, we will focus on platelet purification and on transcript profiling using an Affymetrix microarr

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Artificial Intelligence in Design ’00 group antigens. To efficiently respond to the numerous demands made for hemolytic disease of the newborn cases and polytransfused patients, we designed an inexpensive colorimetric high-throughput method to genotype several blood group antigens rapidly. Three simple steps are required to perform thi

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Lorenzo Mandow,José Luis Pérez De La Cruzor example, recently polytransfused patients, patients with positive direct human antiglobulin tests, and hemolytic disease of the newborn. The traditional polymerase chain reaction techniques are slow and sometimes difficult to carry out and interpret. Thus there is a need for the development and v

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https://doi.org/10.1007/978-94-017-0795-4gy to type 6 common alleles (A1, A2, B, 01, 01 V, and 02) of the ABO blood group system, the high specificity and sensitivity make it suitable also in forensic science. It is more rapid than RFLP and SSCP analysis, resulting in unambiguous interpretation of ABO genotypes and newly discovered mutatio

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Artificial Intelligence in Design ’02method may, however, be adapted for the simultaneous analysis of up to 100 markers (50 biallelic SNPs) in a single reaction. In the method described, the targets of interest are amplified by single-tube multiplex PCR using six primer sets followed by single-tube multiplex allele-specific primer exte