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Titlebook: DNA Vaccines; Methods and Protocol Douglas B. Lowrie,Robert G. Whalen Book 2000 Springer Science+Business Media New York 2000

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1543-1894 d- cerned in the scientific literature of the last decades. However, the present state of affairs appears to date from observations made discreetly in 1988 by Wolff, Malone, Felgner, and colleagues, which were described in a 1989 patent and published in 1990. Quite surprisingly, they showed that ge
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Artificial Intelligence and Soft Computingting the antigenic peptide on their MHC class I molecules. Alternatively, the priming of CTLs may be mediated by professional APC taking up antigen released from muscle cells. Finally, CTL priming may involve direct transfection of APC occurring, albeit at low level, and that the CTLs are activated by the transfected APC.
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Intramuscular Injection of DNA Vaccines in Fish,pecific DNA sequences (CpG motifs), in a particular nucleotide context, act as immunostimulants (.,.). No study has been reported yet assessing these immunostimulatory sequences in fish, but it would be unlikely that the same sequences are effective in all animal species (Weeratna et al., this volume).
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Development of DNA Vaccines for Salmonid Fish,r advantages are the elimination of any possibility of reversion to virulence since the DNA vaccine encodes only a portion of the viral genome, the relative stability of the DNA preparation; the ease of DNA preparation; and, importantly, the elicitation of a robust immune response in fish.
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The Use of Bone Marrow-Chimeric Mice in Determining the MHC Restriction of Epitope-Specific Cytotoxting the antigenic peptide on their MHC class I molecules. Alternatively, the priming of CTLs may be mediated by professional APC taking up antigen released from muscle cells. Finally, CTL priming may involve direct transfection of APC occurring, albeit at low level, and that the CTLs are activated by the transfected APC.
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