| 书目名称 | DNA Vaccines | | 副标题 | Methods and Protocol | | 编辑 | Douglas B. Lowrie,Robert G. Whalen | | 视频video | | | 丛书名称 | Methods in Molecular Medicine | | 图书封面 |  | | 描述 | The field of DNA vaccines has undergone explosive growth in the last few years. As usual, some historical precursors of this approach can be d- cerned in the scientific literature of the last decades. However, the present state of affairs appears to date from observations made discreetly in 1988 by Wolff, Malone, Felgner, and colleagues, which were described in a 1989 patent and published in 1990. Quite surprisingly, they showed that genes carried by pure plasmid DNA and injected in a saline solution, hence the epithet “naked DNA,” could be taken up and expressed by skeletal muscle cells with a low but reproducible frequency. Such a simple methodology was sure to spawn many applications. In a separate and important line of experimentation, Tang, De Vit, and Johnston announced in 1992 that it was indeed possible to obtain humoral immune responses against proteins encoded by DNA delivered to the skin by a biolistic device, which has colloquially become known as the “gene gun. ” The year 1993 saw the publication of further improvements in the me- ods of naked DNA delivery and, above all, the first demonstrations by several groups of the induction of humoral and cytotoxic immune respon | | 出版日期 | Book 2000 | | 版次 | 1 | | doi | https://doi.org/10.1385/1592596886 | | isbn_softcover | 978-1-4899-4182-4 | | isbn_ebook | 978-1-59259-688-1Series ISSN 1543-1894 Series E-ISSN 1940-6037 | | issn_series | 1543-1894 | | copyright | Springer Science+Business Media New York 2000 |
The information of publication is updating
|
|
发表于 2025-3-21 16:57:44
