Titlebook: DNA Topoisomerases; Methods and Protocol Duncan J. Clarke Book 2009 Humana Press 2009 Cellular functions.Chromosom.Chromosome structure.DNA

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Yu An,Qing Ye,Jiulin Guo,Ruihai Dongeps are conducted in orthogonal directions with different concentrations of DNA intercalating agents. These compounds alter the overall shape of the DNA and, thereby, change the relative mobility of individual DNA topoisomers.

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Lecture Notes in Computer Sciencefilter binding assay compares the ability of nicked and supercoiled forms of the circular DNA to compete for the binding of a .H-labeled nicked DNA to the topoisomerase where the enzyme–DNA complexes are quantitated by the retention of the labeled DNA on a nitrocellulose membrane.

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,Dissolution of Double Holliday Junctions by the Concerted Action of BLM and Topoisomerase IIIα,s, which would have the effect of suppressing sister chromatid exchanges and loss of heterozygosity between homologous chromosomes. In this chapter, we provide a detailed experimental protocol for the preparation of an oligonucleotide-based, double Holliday junction substrate and for the biochemical analysis of dissolution in vitro.

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