Titlebook: DNA Topoisomerases; Methods and Protocol Duncan J. Clarke Book 2009 Humana Press 2009 Cellular functions.Chromosom.Chromosome structure.DNA

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Artificial Intelligence XXXVIIIrticularly suited to the study of topoisomerases due to their unique ability to exert precise and straightforward control of the supercoiled state of DNA. Here, we illustrate in a stepwise fashion how the dynamic properties of type IB topoisomerases can be monitored using this technique.

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Artificial Intelligence XXXVIIIbased on the immunoprecipitation of crosslinked chromatin (ChIP, chromatin immunoprecipitation), followed by DNA amplification and hybridization to high-density oligonucleotide arrays (Chip). Comparison of the signal intensities of immunoprecipitated and control fractions provides a measurement of t

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Ada Maria Barone,Emanuela Stagnotromeric chromatin during metaphase. The factors controlling these changes in enzyme distribution are largely unknown. Insight into Topo II dynamics could potentially be derived through genetic approaches in yeast. In practice, however, the small size and limited compaction of yeast chromosomes has

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Studies in Computational Intelligenceion of enzyme poisons specific for type I or type II DNA topoisomerases. Using the topoisomerase I poison camptothecin and the topoisomerase II poison VP16, the precise sites of interaction of these enzymes around the lamin B2 origin have been identified at different points in the cell cycle. The pr

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Studies in Computational Intelligence double-strand breaks (DSBs). DNA damage, particularly induction of DSBs, manifests by phosphorylation of histone H2AX on .139 which is mediated by one of the protein kinases of the phosphoinositide kinase family, namely ATM, ATR, and/or DNA-PK. The presence of .-139 phosphorylated H2AX (γH2AX) is t

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Studies in Computational Intelligencelding the mitotic chromosome. Perturbed Topo II activity causes defects in chromosome segregation due to persistent catenations and aberrant DNA condensation during mitosis. Recently, novel . alleles in the yeast . revealed a checkpoint control that responds to perturbed Topo II activity. Described

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Bahaaeddin Alareeni,Allam Hamdan) for use in studying the dynamics of Topo IIα in living cells. In previous studies, this plasmid was transfected into LLC-Pk cells, a porcine epithelial cell line that remains relatively flat during mitosis. After selection for stable integration, cells were cloned by serial dilution in microwells