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Titlebook: DNA Repair Protocols; Prokaryotic Systems Pat Vaughan Book 2000 Humana Press 2000

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楼主: cobble
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Blutdruck und Blutdruckregulation from DNA. Uracil, either misincorporated in place of thymine or resulting from deamination of cytosine, is removed by a specific glycosylase, uracil-DNA glycosylase, found in prokaryotes and eukaryotes (.,.).
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Detection of DNA Deoxyribophosphodiesterase Activity from DNA. Uracil, either misincorporated in place of thymine or resulting from deamination of cytosine, is removed by a specific glycosylase, uracil-DNA glycosylase, found in prokaryotes and eukaryotes (.,.).
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https://doi.org/10.1007/978-3-642-18507-6.)]. The recombinational repair of a double-stranded DNA (dsDNA) break requires four general steps: (1) processing; (2) homologous pairing; (3) DNA heteroduplex extension; and (4) resolution. Here we describe assays to study aspects of the first two steps.
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Holliday Junction Branch Migration and Resolutionmesophilic or thermophilic. The second involves the purification of Ruv proteins from the thermophile, ., by procedures applicable to the purification of thermostable proteins from a variety of thermophilic genera. The specific assays detail the measurement of branch migration and resolution of the Holliday junction substrate by . Ruv proteins.
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