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Titlebook: DNA Repair Protocols; Prokaryotic Systems Pat Vaughan Book 2000 Humana Press 2000

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书目名称DNA Repair Protocols
副标题Prokaryotic Systems
编辑Pat Vaughan
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: DNA Repair Protocols; Prokaryotic Systems Pat Vaughan Book 2000 Humana Press 2000
描述When setting out to decide on the content of DNA Repair Protocols: Prokaryotic Systems, I was conscious of the need to portray the vast array of pathways and enzymatic activities that are part of the discipline of DNA repair. In addition to the classical DNA repair activities, I wanted to convey the significant interest that has been generated in recent years in the use of the proteins and repair systems as research tools, much like the use of restriction enzymes over the last few decades. Therefore, in addition to chapters deta- ing protocols for investigating specific repair activities, I have included s- eral chapters in this book on the applied use of DNA repair proteins and systems. The many years of research on bacterial DNA repair systems have allowed us to really understand the majority of DNA repair pathways in bac- rial cells. Building on this knowledge, research has lead to major advances in understanding mammalian DNA repair and uncovered its links to human d- ease, such as DNA mismatch repair and colon cancer, nucleotide excision repair and xeroderma pigmentosum, DNA helicase function in Bloom’s s- drome, and so on. Such have been the advances that Science magazine ide
出版日期Book 2000
版次1
doihttps://doi.org/10.1385/1592590683
isbn_softcover978-1-61737-112-7
isbn_ebook978-1-59259-068-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2000
The information of publication is updating

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Aneurysmen und dilatative Angiopathied kinetics are desirable. Instrumentation providing such capability is a worthwhile complement to classic assays, such as filter binding and electrophoretic mobility shift assays. Here, we describe techniques for studying the interaction of MutS and MutL with immobilized DNA using surface plasmon re
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https://doi.org/10.1007/978-3-642-74518-8imited technician effort; (3) low cost: for wide-spread use in both research and clinical diagnostics, low-cost and easy availability of both equipment and reagents is crucial; (4) no gels: this requirement is primarily to meet the high throughput requirement; (5) no radioactivity: given the problem
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https://doi.org/10.1007/978-3-642-68106-6esolvase enzymes, T4 endonuclease VII (.) and T7 endonuclease I (.), both bacteriophage enzymes with similar in vivo functions. The one-step binding and cleavage reaction replace the two-step CCM procedure that uses different chemicals in each stage that are not active in the same buffer and thus re
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Book 2000jor advances in understanding mammalian DNA repair and uncovered its links to human d- ease, such as DNA mismatch repair and colon cancer, nucleotide excision repair and xeroderma pigmentosum, DNA helicase function in Bloom’s s- drome, and so on. Such have been the advances that Science magazine ide
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MutS–DNA Interactions and DNase Protection Analysis with Surface Plasmon Resonanced kinetics are desirable. Instrumentation providing such capability is a worthwhile complement to classic assays, such as filter binding and electrophoretic mobility shift assays. Here, we describe techniques for studying the interaction of MutS and MutL with immobilized DNA using surface plasmon re
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1064-3745 smatch repair and colon cancer, nucleotide excision repair and xeroderma pigmentosum, DNA helicase function in Bloom’s s- drome, and so on. Such have been the advances that Science magazine ide978-1-61737-112-7978-1-59259-068-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
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