Titlebook: DNA Repair Protocols; Daryl S. Henderson Book 2006 Humana Press 2006 DNA.DNA replication.Ligation.PCR.Polyacrylamid-Gelelektrophorese.Quan

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Michael Hristov,Christian Weberor ultraviolet light to challenge cells to repair the induced damage, and chromosome aberrations as a biomarker to indicate DNA repair proficiency. The assay was used successfully to demonstrate base- and nucleotide-excision repair deficiency in certain polymorphic DNA repair genes, namely . 751Gln

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Clinical Classification of Arterial Graftsced genomic instability. Perhaps the best characterized is the dynamic production of chromosomal rearrangements in some clonally expanded cells surviving irradiation. In this chapter we provide the protocols for irradiation, cell culture, chromosome analysis, and characterization of the status of ge

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M. Durairaj MS, MCh,B. Buxton MBBS, FRACStigations over the last 40 yr. Recent studies have identified several components of this checkpoint response and there is strong interest in its biochemical characterization. Helpful for the delineation of the mechanism of the S-phase checkpoint is the observation that factors inhibiting DNA replica

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E. Berreklouw MD, PhD,G. -W. He MD, PhD, DScde of cell death. This chapter describes methods to label . DNA strand breaks with fluorochromes for detection by flow or laser scanning cytometry. By staining DNA with a fluorochrome of another color, cellular DNA content is measured concurrently and the bivariate analysis of such a data reveals DN