Titlebook: DNA Repair Protocols; Daryl S. Henderson Book 2006 Humana Press 2006 DNA.DNA replication.Ligation.PCR.Polyacrylamid-Gelelektrophorese.Quan

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Use of Gene Targeting to Study Recombination in Mammalian Cell DNA Repair Mutants,seases. Gene targeting approaches are also useful for studying the mechanisms of homologous recombination. We have developed gene targeting methods that we have specifically used to investigate the mechanisms of recombination in cultured mammalian cells. In this chapter, we describe the generation o

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painkillers

Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian ication of long DNA targets. This assay has been extensively used to measure the integrity of both nuclear and mitochondrial genomes exposed to different genotoxins, and has proved particularly valuable in identifying reactive oxygen species-mediated mitochondrial DNA (mtDNA) damage. QPCR can be use

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Measuring the Formation and Repair of DNA Damage by Ligation-Mediated PCR, the genome is modulated by the DNA sequence, by DNA methylation patterns, by the transcriptional status of the locus, and by chromatin proteins associated with the DNA. The only method currently available to allow a precise sequence mapping of DNA lesions in mammalian cells is the ligation-mediated

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Immunochemical Detection of UV-Induced DNA Damage and Repair,eous genetic damage has developed. This, in turn, has increased interest in the cellular mechanisms responsible for tumorigenesis, and the need to develop experimental methodologies to investigate these mechanisms. DNA represents a most important cellular target for ultraviolet radiation (UVR), lead

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A Dot-Blot Immunoassay for Measuring Repair of Ultraviolet Photoproducts,t light (UV-C)—pyrimidine-pyrimidone 6-4 photoproducts ([6-4]PPs) and cyclobutane pyrimidine dimers (CPDs). DNA extracted from irradiated cells is applied to a nitrocellulose dot-blot and quantified using an enzyme-conjugated secondary antibody and a color assay. Though the polyclonal antiserum cont

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analogous

DNA Damage Quantitation by Alkaline Gel Electrophoresis,gents, as well as the ability of cells to repair such damages. Quantitative gel electrophoresis of experimental DNAs along with DNA length standards, imaging the resulting dispersed DNA and calculating the population average length allows accurate measurement of lesion frequencies. Number average le

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DNA Repair Protocols978-1-59259-973-8Series ISSN 1064-3745 Series E-ISSN 1940-6029