Titlebook: DNA Modifications; Methods and Protocol Alexey Ruzov,Martin Gering Book 2021 The Editor(s) (if applicable) and The Author(s), under exclusi

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Immunohistochemical Detection of Modified Cytosine Bases in Rodent Brainlevels is possible through use of immunohistochemistry. In this chapter we describe an adaptable method of brain tissue collection and immunohistochemical staining that allows for detection of 5hmC in mouse or rat brain, meaning that the method can be applied to many rodent models of CNS diseases and disorders.

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Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260167.jpg

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DNA Modifications978-1-0716-0876-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/978-3-031-45445-5 methods such as dot blotting and liquid chromatography-mass spectrometry (LC-MS). Herein, we describe a high throughput capillary gel electrophoresis assay for monitoring the in vitro oxidation of 5mC by TET. The method is rapid and quantitative, and can serve as a powerful tool in mechanistic studies of TET.

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Evidence for Noncytosine Epigenetic DNA Modifications in Multicellular Eukaryotes: An Overviewd its oxidized derivatives in the regulation of gene expression are relatively well attested and extensively explored, a number of recent studies imply that noncytosine DNA modifications may also convey specific biological functions and act as “epigenetic” marks in multicellular organisms. Here we r

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Mass Spectrometry-Based Analysis of DNA Modifications: Potential Applications in Basic Research and generated DNA nucleobase modifications. It is regarded as a gold standard for such analyses. Here, we consider the requirements for reliable identification and quantification of DNA adducts/modifications, whether endogenously derived or not, and discuss how their quantification can provide informat