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Titlebook: DNA Modifications; Methods and Protocol Alexey Ruzov,Martin Gering Book 2021 The Editor(s) (if applicable) and The Author(s), under exclusi

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发表于 2025-3-21 16:12:30 | 显示全部楼层 |阅读模式
书目名称DNA Modifications
副标题Methods and Protocol
编辑Alexey Ruzov,Martin Gering
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: DNA Modifications; Methods and Protocol Alexey Ruzov,Martin Gering Book 2021 The Editor(s) (if applicable) and The Author(s), under exclusi
描述This book provides an overview of methods and experimental protocols that are currently used to analyze the presence and abundance of non-canonical DNA nucleotides in different biological systems. Focusing particularly on the newly discovered and less studied DNA modifications that are enzymatically produced and are likely to play specific roles in various biological processes, the volume explores chromatography- and mass spectrometry-based techniques for the detection and quantification of DNA modifications, antibody-based approaches to study their spatial distribution in different cells and tissues, and methods to analyze their genomic distribution with the help of bioinformatics tools that interrogate the corresponding datasets. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and comprehensive, .DNA Modifications: Methods and Protocols. serves as an ideal guide to research scientists and PhD students in this rapidly develop
出版日期Book 2021
关键词Non-canonical DNA nucleotides; DNA demethylation; Mass Spectrometry; Antibody-based techniques; Bioinfor
版次1
doihttps://doi.org/10.1007/978-1-0716-0876-0
isbn_softcover978-1-0716-0878-4
isbn_ebook978-1-0716-0876-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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MS Analysis of DNA Modifications in Urinary/Body Fluidswo-dimensional ultraperformance liquid chromatography with tandem mass spectrometry (2D-UPLC-MS/MS) and high-performance liquid chromatography coupled with gas chromatography and mass spectrometry (HPLC/GC/MS).
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Lecture Notes in Computer Science reach. Here, we review the techniques currently available for profiling DNA modifications in mammals, discuss their strengths and weaknesses, and speculate on the future direction of DNA modification profiling technologies.
发表于 2025-3-22 16:22:34 | 显示全部楼层
https://doi.org/10.1007/978-3-319-16277-5ced a similar rate of development and is often a bottleneck to chemical analysis. Here we present a protocol for identification and quantification of cytosine modifications that combines a robust and efficient method to generate single nucleosides from genomic DNA samples followed by direct LC-MS/MS analysis.
发表于 2025-3-22 19:11:54 | 显示全部楼层
Mapping DNA Methylation in Mammals: The State of the Art reach. Here, we review the techniques currently available for profiling DNA modifications in mammals, discuss their strengths and weaknesses, and speculate on the future direction of DNA modification profiling technologies.
发表于 2025-3-22 23:54:00 | 显示全部楼层
Liquid Chromatography–Mass Spectrometry Analysis of Cytosine Modificationsced a similar rate of development and is often a bottleneck to chemical analysis. Here we present a protocol for identification and quantification of cytosine modifications that combines a robust and efficient method to generate single nucleosides from genomic DNA samples followed by direct LC-MS/MS analysis.
发表于 2025-3-23 01:52:49 | 显示全部楼层
Book 2021A nucleotides in different biological systems. Focusing particularly on the newly discovered and less studied DNA modifications that are enzymatically produced and are likely to play specific roles in various biological processes, the volume explores chromatography- and mass spectrometry-based techn
发表于 2025-3-23 06:12:06 | 显示全部楼层
Sihem Mesnager,Ferruh Özbudak,Ahmet Sınakdroxymethyluracil (5hmU) and N6-methyladenine (6mA), and suggest potential explanations for inconsistencies in the currently available data on abundance and potential biological roles of these DNA modifications in mammals.
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