Titlebook: DNA Methylation; Methods and Protocol Jörg Tost Book 2009Latest edition Humana Press 2009 DNA.Epigenetics.Epigenomics.Gene-specific methyla

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Jörg TostProvides an easily accessible reference volume for DNA methylation technologies as written by leading experts in the field.Focuses on validated and breakthrough innovations for all major questions of

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Quantification of Global DNA Methylation by Capillary Electrophoresis and Mass Spectrometrytection and quantification of 5-methyl 2′-deoxycytidine in genomic DNA is performed using both high-performance capillary electrophoresis (HPCE) with UV–Vis detection or liquid chromatography with electrospray ionization mass spectrometric detection (LC-ESI/MS). Treatment of genomic DNA with a ribon

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Methyl Group Acceptance Assay for the Determination of Global DNA Methylation Levelsated with genomic hypomethylation and localized hypermethylation of tumor suppressor genes. To reverse methylation-induced gene repression, DNA hypomethylating agents are currently in clinical trials for various malignancies, with two of these now approved for the therapy of myelodysplastic syndrome

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Immunodetection Arrayon direct immunodetection of 5′ methylcytosines (5′ mCs) without the need for bisulfite treatment utilizing a microarray format. This system is designed for the application of immunofluorescence using a monoclonal antibody that specifically recognizes 5′ mC in single-stranded DNA hybridized to oligo

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Methylated DNA Immunoprecipitation (MeDIP)randomly sheared by sonication and immunoprecipitated with a monoclonal antibody that specifically recognizes 5-methylcytidine. The resulting enrichment of methylated DNA in the immunoprecipitated fraction can be determined by PCR to assess the methylation state of individual regions. Alternatively,

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The Help Assaygment enrichment by ligation-mediated PCR (HELP) is an example of the use of such representations to study cytosine methylation in the genome. The HELP assay differs from most other assays testing cytosine methylation because of its positive representation of hypomethylated DNA in the genome, wherea

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