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Titlebook: DNA Methylation; Methods and Protocol Jörg Tost Book 2009Latest edition Humana Press 2009 DNA.Epigenetics.Epigenomics.Gene-specific methyla

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发表于 2025-3-21 18:40:41 | 显示全部楼层 |阅读模式
书目名称DNA Methylation
副标题Methods and Protocol
编辑Jörg Tost
视频video
概述Provides an easily accessible reference volume for DNA methylation technologies as written by leading experts in the field.Focuses on validated and breakthrough innovations for all major questions of
丛书名称Methods in Molecular Biology
图书封面Titlebook: DNA Methylation; Methods and Protocol Jörg Tost Book 2009Latest edition Humana Press 2009 DNA.Epigenetics.Epigenomics.Gene-specific methyla
描述.Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace. Due to this fact, DNA Methylation: Methods and Protocols, Second Edition completely revises, updates, and expands upon the popular first edition with the most current novel techniques, easier to use and more refined by the tested experience of leading experts. This revision reflects contemporary study of the subject: the analysis of gene-specific DNA methylation patterns has been complemented by genome-wide approaches, and epigenomics takes a central place. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, DNA Methylation: Methods and Protocols, Second Edition is the perfect resource for scientists who wish to further our understanding of the molecular processes that determine the genomic DNA methylation landscape and for those who
出版日期Book 2009Latest edition
关键词DNA; Epigenetics; Epigenomics; Gene-specific methylation; Microarray; PCR; Termination; Whole genome analys
版次2
doihttps://doi.org/10.1007/978-1-59745-522-0
isbn_softcover978-1-61737-938-3
isbn_ebook978-1-59745-522-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2009
The information of publication is updating

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Kostenermittlungen nach DIN 276-noise ratio and to minimize autofluorescence. These methodological improvements allow for the direct detection of 5′ mC in genomic DNA hybridized to microarrays without prior PCR amplification with high analytical sensitivity.
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Quantification of Global DNA Methylation by Capillary Electrophoresis and Mass Spectrometryonstrated to be highly specific and sensitive, being useful for the rapid quantification of the degree of global DNA methylation and its exploitation for the analysis of poorly purified and/or concentrated DNA samples, such as tumor biopsies.
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Immunodetection Array-noise ratio and to minimize autofluorescence. These methodological improvements allow for the direct detection of 5′ mC in genomic DNA hybridized to microarrays without prior PCR amplification with high analytical sensitivity.
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Book 2009Latest editiono this fact, DNA Methylation: Methods and Protocols, Second Edition completely revises, updates, and expands upon the popular first edition with the most current novel techniques, easier to use and more refined by the tested experience of leading experts. This revision reflects contemporary study of
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