Titlebook: DNA Cloning and Assembly; Methods and Protocol Sunil Chandran,Kevin W. George Book 2020 Springer Science+Business Media, LLC, part of Sprin

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Orientierung an Gebrauchswertento use, efficient, and allow users to assemble multigene constructs by performing a series of one-pot assembly steps, starting from libraries of cloned and sequenced parts. The efficiency of these systems also facilitates the generation of libraries of construct variants. We describe here a protocol

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Computer — Mega-Maschine oder Werkzeugpe IIS restriction enzyme for hierarchical modular DNA assembly. This offers great flexibility in the design of the assembly experiment and simplicity of execution. Here we describe a protocol for hierarchical assembly of large DNA constructs from modular DNA parts using the MetClo vector set, a set

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Die ökonomische Sicht auf die Arbeitbly methods. Firstly, coding sequences are assembled with upstream and downstream sequences via overhangs corresponding to start and stop codons, avoiding unwanted ‘scars’ in assembled constructs at coding sequence boundaries. Scars at these crucial, sensitive locations can affect mRNA structure, ac

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