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Titlebook: DNA Cloning and Assembly; Methods and Protocol Sunil Chandran,Kevin W. George Book 2020 Springer Science+Business Media, LLC, part of Sprin

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发表于 2025-3-21 18:22:14 | 显示全部楼层 |阅读模式
书目名称DNA Cloning and Assembly
副标题Methods and Protocol
编辑Sunil Chandran,Kevin W. George
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: DNA Cloning and Assembly; Methods and Protocol Sunil Chandran,Kevin W. George Book 2020 Springer Science+Business Media, LLC, part of Sprin
描述.This volume provides a comprehensive collection of DNA assembly protocols that will prove useful for any researcher interested in molecular cloning, synthetic biology, or DNA manipulation. Chapters will guide readers through computational tools to design and track the construction of DNA assemblies, workflows that enable high-throughput assembly of DNA constructs, standardized toolkits and protocols for DNA assembly, and combinatorial solutions that enable the construction and optimization of entire metabolic pathways. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .DNA Cloning and Assembly: Methods and Protocols .aims to ensure successful results in the further study of this vital field..
出版日期Book 2020
关键词Gibson assembly; DNA assembly; MODAL/BASIC; gene synthesis; Biobrick
版次1
doihttps://doi.org/10.1007/978-1-0716-0908-8
isbn_softcover978-1-0716-0910-1
isbn_ebook978-1-0716-0908-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2020
The information of publication is updating

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Generation of MoClo Standard Parts Using Golden Gate Cloning for amplification, (3) performing PCR amplification, (4) cloning of the fragments using Golden Gate cloning, and finally (5) sequencing of the part. For large basic parts, it is preferable to first clone subparts as intermediate level −1 constructs. These subparts are sequenced individually and are
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Design and Implementation of Multi-protein Expression Constructs and Combinatorial Libraries using Sw vector is required in order to assemble constructs for any new destination context, such as a new organism or genomic location. This should facilitate more rapid and convenient development of engineered metabolic pathways for diverse nonmodel organisms in order to exploit their applied potential.
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BASIC: A Simple and Accurate Modular DNA Assembly Methodenter a subsequent BASIC assembly without the need for reformatting or changes to the workflow. This unique idempotent cloning mechanism allows for the assembly of constructs in multiple, conceptionally simple hierarchical rounds. Combined with its high accuracy and robustness, this makes BASIC a ve
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Die ökonomische Sicht auf die Arbeitw vector is required in order to assemble constructs for any new destination context, such as a new organism or genomic location. This should facilitate more rapid and convenient development of engineered metabolic pathways for diverse nonmodel organisms in order to exploit their applied potential.
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