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Titlebook: Cytoskeleton Proteins; A Purification Manua Gerhard Isenberg Book 1995 Springer-Verlag Berlin Heidelberg 1995 Actin.Aktin.Microfilaments.Mi

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Peter Biro,Manfred Abel,Thomas PaschAmmonium sulfate precipitation is an appropriate method to fractionate proteins prior to purification by chromatographic procedures. It is also used for concentrating protein samples. Ultra-pure ammonium sulfate (e.g. supplied by Schwarz-Mann) is recommended. AS may be added as solid compound or by dilution from a saturated stock solution.
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Peter Biro,Manfred Abel,Thomas PaschA 240 KDa filamin equivalent protein from Dictyostelium discoideum, which crosslinks and bundles actin filaments at the cell cortex.
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Anästhesie bei seltenen ErkrankungenA 75 kDa spherical molecule of ~9 nm in diameter, probably dimeric (~150 kDa) in its native form. A heat unstable, non-calmodulin binding MAP of sea urchin eggs.
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https://doi.org/10.1007/978-3-662-05712-4A multigene family of diverse 40–80 KDa type I (acidic) and type II (basic) polypeptides ranging from pI 4.8–8.0, constituating intermediate filaments (IF) in various epitelial cells and tissues.
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Peter Biro,Manfred Abel,Thomas PaschA heterodimeric calmodulin binding protein from the erythrocyte membrane skeleton composed of α (103 kDa) and β (97 kDa) subunits, also present in vertebrate brain.
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General Lab EquipmentThe following general equipment is required for routine cytoskeleton protein purifications:
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Preparation of BuffersIn general, buffers are made up according to two methods:
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