Titlebook: Cytochrome P450 Protocols; Ian R. Phillips,Elizabeth A. Shephard Book 19981st edition Springer Science+Business Media New York 1998

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揭穿真相

噱头

Spectral Analyses of Cytochromes P450, heme found in b-type cytochromes, like cytochrome b, hemoglobin, and myoglobm. This type of heme prosthetic group can be removed from the protein by acidic acetone or by alkali and pyridine. There are a number of methods that can be used for spectrophotometrrc analysis of hemoproteins, each useful

gene-therapy

Purification of Cytochromes P450,quisite to many types of investigation, purified enzymes are still required for studies on structure and mechanism. Purified enzymes are also needed for preparation of antibodies, as standards in assays, and for other purposes that may often be overlooked.

paradigm

FECK

FECK

Oratory

Cytochrome P450 Reconstitution Systems,ucturally diverse xenobrotrc chemicals such as drugs, toxic chemicals, and carcinogens, as well as endobiottc chemicals including steroids, fatty acids, fat-soluble vitamins, and prostaglandins (.). Major cytochrome P450 enzymes in human liver microsomes identified to date mclude CYPlA2,2A6,2B6,2C8,

DEI

Catalytic Assays for Human Cytochrome P450,ny P450 enzymes have been isolated and identified in liver and other tlssues, including kidney, lung, intestines, and brain. Although these enzymes have a high degree of similarity in their ammo-acid sequences, many of them are subject to differential regulation and have distinct catalytic functions

precede

Enzymatic Analysis of cDNA-Expressed Human CYP1A1, CYP1A2, and CYP1B1 with 7-Ethoxyresorufin as Suber (., .), but its expression is readily detectable in lung (., .). By contrast, CYP1A2 is constitutively expressed in human liver (., .) and is absent in lung (., .). CYP1B1 is primarily an extrahepatic P450, as suggested by the findmg that CYP1B1 mRNA is present in much greater abundance in tissue