Titlebook: Cystic Fibrosis; Diagnosis and Protoc Margarida D. Amaral,Karl Kunzelmann Book 2011 Springer Science+Business Media, LLC 2011 CF.Genomics.H

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Identification and Quantification of Mucin Expressioncial considerations and methodology. We describe methods for the general quantification of heavily glycosylated proteins as the mucins using dot/slot blot. We also describe the separation of the mucins by gel electrophoresis and the identification with specific antibodies on Western blot and by proteomics.

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Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells cells on microporous membrane at the air–liquid interface. Here, we describe approaches to study differentiation as well as repair of the hAECs by using a commercially available airway cell culture model called MucilAir™.

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Problem Statement and Preliminariesantibodies and optimized staining protocols. CFTR tagged by green fluorescent protein can be employed to study trafficking in live cells. Tagging of CFTR with an extracellular epitope permits detection exclusively at the cell surface and subsequent chasing allows visualization of endocytic trafficking.

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Anime from Akira to Princess Mononokely involved in the basic defect(s). New global strategies and tools identifying gene products responsible for the basic cellular defect(s) in CF pathophysiology currently being performed are presented here. These include high-content screens to determine proteins affecting function and trafficking of CFTR and ENaC.

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Book 2011rasp and use the already wide and still growing range of diverse methods currently employed to study CF so as to understand it in its multidisciplinary nature.  .Cystic Fibrosis: Diagnosis and Protocols. aims to provide the CF research community and related researchers with a very wide range of high

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Video-Based Performance Captureher transporters. The modulation of epithelial Na. channel (ENaC) function serves as a prime example of regulatory function of the CFTR. In this chapter, we will briefly describe an integrated protocol consisting of biochemical and electrophysiological approaches to study the regulation of ENaC by CFTR.

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Animation und Interaktion im WWWlso demonstrate that these methods are also applicable in novel ways to probe the ultrastructure of the airways including the establishment of pH gradients and the ability of the apical membrane glycocalyx in excluding larger molecules from the cell surface.

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https://doi.org/10.1007/978-3-642-48790-3ral analysis of secretion rates. The composition and properties of the mucus (e.g., solids, viscosity, pH) can also be determined. These methods have now been applied to mice, ferrets, cats, pigs, sheep, and humans, with a main goal of comparing gland secretion in control and CFTR-deficient humans and animals.

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