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Titlebook: Cystic Fibrosis; Diagnosis and Protoc Margarida D. Amaral,Karl Kunzelmann Book 2011 Springer Science+Business Media, LLC 2011 CF.Genomics.H

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Imaging CFTR Protein Localization in Cultured Cells and Tissuesantibodies and optimized staining protocols. CFTR tagged by green fluorescent protein can be employed to study trafficking in live cells. Tagging of CFTR with an extracellular epitope permits detection exclusively at the cell surface and subsequent chasing allows visualization of endocytic trafficking.
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Animation, Embodiment, and Digital Media cells on microporous membrane at the air–liquid interface. Here, we describe approaches to study differentiation as well as repair of the hAECs by using a commercially available airway cell culture model called MucilAir™.
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Problem Statement and Preliminariesding this link, and this series of chapters details the recent progress that has been made in understanding the interplay between ion transport activity and innate lung defense, and the initiation of CF lung pathophysiology.
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