Titlebook: Chromatin Immunoprecipitation; Methods and Protocol Franziska Greulich Book 2024 The Editor(s) (if applicable) and The Author(s), under exc

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Introduction to Macroporous Cryogelsunoprecipitation (ChIP-Seq), requires only a limited amount of cells as starting material. RNA sequencing (RNA-Seq) reveals the presence and quantity of RNA in a biological sample, describing the continuously changing cellular transcriptome. The integrated analysis of transcriptional activity, histo

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https://doi.org/10.1007/978-1-59745-582-4tiation, the distribution of histone modifications is remodeled, resulting in cell type–specific patterns. In the past, their study was limited to abundant cell types that could be purified in necessary numbers. However, studying these cell type–specific dynamic changes in heterogeneous in vivo sett

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Stuart R. Gallant,Vish Koppaka,Nick Zecherlese-seq data at the nucleotide level. We introduce an assay termed cross-link (XL)-DNase-seq, designed to capture chromatin interactions of dynamic TFs. A mild cross-linking step in XL-DNase-seq improves the detection of DNase-based footprints of dynamic TFs. The footprint strengths and detectability

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Adam Charlton,Michael Zachariounding locations in vivo, does not require antibodies or fixation, and provides genome-wide coverage at near nucleotide resolution..The core of this method is an MNase fusion of the target protein, which allows it, when triggered by calcium exposure, to cut DNA at its binding sites and to generate sm

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