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Titlebook: Chromatin Immunoprecipitation; Methods and Protocol Franziska Greulich Book 2024 The Editor(s) (if applicable) and The Author(s), under exc

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Pablo Fossa,Cristian Cortés-Riverantrol of specific transcriptional factors or co-regulators at loci-specific or pan-genomic levels..Here we provide a reliable method for processing ChIP from adipocytes or frozen adipose tissue collection, isolation of nuclei, cross-linking of protein-DNA complexes, chromatin shearing, immunoprecipi
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https://doi.org/10.1007/978-3-031-31709-5nteractions in the last decade. ChIP-seq technology became standard both experimentally and computationally. This chapter presents a core workflow that covers data processing and initial analytical steps of ChIP-seq data. We provide a step-by-step protocol of the commands as well as a fully assemble
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https://doi.org/10.1007/978-3-642-79726-2of defined genes. In this study, we developed a novel method for capturing the proteins that bind to certain chromatin fragments or DNA sequences, which is called reverse chromatin immunoprecipitation (R-ChIP). This technology uses a set of specific DNA probes labeled with biotin to isolate chromati
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