Titlebook: Calcium Signaling; Methods and Protocol Caroline M. Gorvin Book 2025 The Editor(s) (if applicable) and The Author(s), under exclusive licen

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书目名称Calcium Signaling影响因子(影响力)




书目名称Calcium Signaling影响因子(影响力)学科排名




书目名称Calcium Signaling网络公开度




书目名称Calcium Signaling网络公开度学科排名




书目名称Calcium Signaling被引频次




书目名称Calcium Signaling被引频次学科排名




书目名称Calcium Signaling年度引用




书目名称Calcium Signaling年度引用学科排名




书目名称Calcium Signaling读者反馈




书目名称Calcium Signaling读者反馈学科排名

尾巴

Bioluminescence Resonance Energy Transfer (BRET) Assay to Measure Gq Recruitment to the Ghrelin Recements, offering a robust protocol to examine the temporal aspects of Gq signaling in live cells. This methodology facilitates a nuanced understanding of Gq-mediated cellular responses, but also provides researchers with a valuable tool for dissecting other related signaling pathways.

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chronicle

Measuring Calcium Levels in Bone-Resorbing Osteoclasts and Bone-Forming Osteoblastsentiation and activity. Here, we describe the differentiation of human osteoclasts and osteoblasts in vitro and provide common methods to determine cell differentiation and activity. We then describe protocols for measuring intracellular calcium in these cells using Fura2-AM.

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Parallel Arrays of Binary Actuatorsocesses. Moreover, changes in activity can be observed in real time in response to physiological interventions or disease-causing insults. In this chapter, we will provide a detailed protocol for performing this powerful research technique.

军火

Book 2025ing with chapters on cellular assays to assess calcium signaling, the book continues with sections on imaging methods, methods to assess calcium within primary cilia, the endoplasmic reticulum, and the mitochondria, as well as tissue-specific calcium signaling methods, including the establishment of

CURT

https://doi.org/10.1007/978-94-011-4120-8 valuable tool for those conducting pharmacological investigations and compound screening targeting this critical cellular pathway. This protocol chapter covers experimental setup, sample preparation, and data analysis, providing researchers with an in-depth guide to explore the pharmacology of Gq-coupled receptors.

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Veljko Milenkovic,Paul H. Milenkovictroduces various methods for investigating calcium signaling cascades in cells as well as in cilia (thin hairlike projections) specifically, where calcium signaling is triggered by different cilial manipulation techniques.