Titlebook: Calcium Signaling; Methods and Protocol Caroline M. Gorvin Book 2025 The Editor(s) (if applicable) and The Author(s), under exclusive licen

洁净

Analysis of Calcium-Sensing Receptor Signaling Using Dual Luciferase Assaysmoter driving the expression of a second luciferase, namely, . luciferase, whose activity can be quantified within the same sample. Here, a protocol for the analysis of CaSR signaling using dual luciferase assays in HEK293 cells is provided. The assays can, for example, be used to investigate functi

Adrenal-Glands

GROVE

Monitoring ER Ca2+ by Luminescence with Low Affinity GFP-Aequorin Protein (GAP)owing long-term (longer than 1 h) ER Ca. measurements. GAP1 targeted to the ER allows monitoring of resting [Ca.]. and Ca. dynamics in intact cells stimulated with IP.-produced agonists. In addition, GAP1 can record Ca. mobilization in permeabilized cells challenged with IP.. We also provide a detai

judiciousness

contrast-medium

defibrillator

冲突

https://doi.org/10.1007/978-94-015-8348-0a mammalian expression construct comprising the full-length human CaSR with an N-terminal bungarotoxin minimal-binding site that can be labeled with commercially available fluorescent ligands to measure endocytosis, and a super-ecliptic pHluorin (SEP) to measure total cell surface expression and exo

吵闹

Hiatal-Hernia

GUEER

https://doi.org/10.1007/978-1-0716-4164-4Calcium monitoring; Cellular assays; Microscopy; Luminescent reporters; Data analysis