Titlebook: Cryptosporidium; Methods and Protocol Jan R. Mead,Michael J. Arrowood Book 2020 Springer Science+Business Media, LLC, part of Springer Natu

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deviate

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Preparation of , DNA for Whole Genome Sequencing,able culture systems to generate sufficient DNA that is required. Here, we provide a method for the preparation of sufficient, high-quality . DNA for WGS from feces, incorporating parasite purification stages and an optional whole genome amplification step.

Medicaid

High-Throughput Screening of Drugs Against the Growth of , In Vitro by qRT-PCR,ut screening (HTS) of drugs against . in vitro was impractical by the labor-intensive traditional assays. Here we describe a simplified quantitative RT-PCR assay suitable for HTS of compounds and for evaluating drug efficacy against the growth of . in vitro.

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malign

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Cryptosporidium978-1-4939-9748-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Inventory and Monitoring Studies, half have been reported from humans, and most human cases of cryptosporidiosis are caused by . or .. Here we describe a nested PCR for the detection of all . spp. that can then be differentiated by sequencing the PCR amplicons, and a duplex, real-time PCR for the simultaneous detection and differentiation of . and ..