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Titlebook: Cryptosporidium; Methods and Protocol Jan R. Mead,Michael J. Arrowood Book 2020 Springer Science+Business Media, LLC, part of Springer Natu

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Sampling Strategies: Applications,development and commercialization of improved enzyme immunosorbent assays (EIA) for coproantigen detection provided an automatable method for mass testing, and rapid diagnostics when incorporated onto a cartridge format. Similarly, immunochromatographic lateral flow assays (ICLF) enable rapid diagno
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A Practical Guide to Study Design,nd ultrafiltration in the field and ultrafilters are processed in a laboratory. Microbes recovered from the filters are further concentrated and subjected to . isolation or nucleic acid extraction methods for the detection of . oocysts or . DNA.
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Sampling Strategies: Applications,s surface and apical complex antigens are posttranslationally modified with O- and N-linked glycans. This presents a significant challenge to understanding the functions of these antigens and the immune responses to them. Isolation of large amounts of native antigen from . oocysts is expensive and i
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Sampling Strategies: Fundamentals,wn utility in epidemiologic investigations identifying case linkages and contamination sources that could not be determined with traditional epidemiologic tools. The standardized method for . genotyping from stool specimens described here comprises multiple individual protocols to amplify and sequen
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Inventory and Monitoring Studies,able culture systems to generate sufficient DNA that is required. Here, we provide a method for the preparation of sufficient, high-quality . DNA for WGS from feces, incorporating parasite purification stages and an optional whole genome amplification step.
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