Titlebook: Cryopreservation of Mammalian Gametes and Embryos; Methods and Protocol Zsolt Peter Nagy,Alex C. Varghese,Ashok Agarwal Book 2017 Springer

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Chapter 7 Human Epididymal and Testicular Sperm Cryopreservationdirectly have been used to benefit patients suffering from obstructive and nonobstructive azoospermia. Various studies have demonstrated comparable fertilization, ongoing pregnancy, and implantation rates when fresh and frozen-thawed epididymal sperms were used for ICSI [.]. Injection of fresh and f

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Chapter 8 Human Oocytes Slow-Rate Freezing: Methodologymoral issues related to embryo cryopreservation (Coticchio et al., Human Fertil (Camb) 4:152–157, 2001; Tucker et al., Eur J Obstet Gynecol Reprod Biol 113: 524–527, 2004) and maintains reproductive potential in women with diseases or conditions that may compromise reproductive capacity. Here, we de

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Chapter 14 Establishing an Oocyte Cryobank Networkto elective and medically driven reasons for oocyte cryostorage for fertility preservation, donor oocyte cryobanking is beginning to gain traction, potentially replacing fresh oocyte donation in assisted reproduction. Donor “egg banking,” while not totally analogous to donor sperm banking, does prov

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Chapter 9 Slow Freezing and Thawing of Human Cleavage Stage Embryos risks associated with multiple pregnancy while maintaining high cumulative pregnancy rates from single oocyte collection cycles. In this chapter, we describe a simple slow freezing procedure for human early cleavage stage embryos that results in a high proportion of post-thaw embryos surviving and retaining their implantation potential.