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Titlebook: Cryopreservation of Mammalian Gametes and Embryos; Methods and Protocol Zsolt Peter Nagy,Alex C. Varghese,Ashok Agarwal Book 2017 Springer

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Zsolt Peter Nagy,Alex C. Varghese,Ashok AgarwalIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
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Chapter 4 Role of Antioxidants and Antifreeze Proteins in Cryopreservation/Vitrification and function of oocytes and ovarian tissues (OT) in animal models. In this chapter, the experimental protocols for the use of antioxidants and antifreeze proteins in cryopreservation/vitrification are described.
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Chapter 5 Slow Freezing of Human Sperme a crucial impact on the reproductive outcome of assisted reproduction technologies. Here, we present, explain, and describe the slow freezing method for preserving human spermatozoa, which is currently the most commonly used freezing technique in most clinical andrology laboratories.
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Chapter 6 Technology of Aseptic Cryoprotectant-Free Vitrification of Human ICSI Spermatozoaillaries (for ICSI or IVF in microvolume). Spermatozoa, vitrified by this technology, are free from seminal plasma owing to swim-up procedure preceding vitrification and are free from permeable cryoprotectants. They are ready for further use immediately after warming without any additional treatment.
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Sui Yu,Lichen Zhang,Lixia Li,Xiaoming WangSuccess in cryopreservation of ovarian tissue (OT) in animal models has led to develop efficient cryo-technologies for human ovarian tissue. In this chapter, cryopreservation protocols developed for animal experiments are described.
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Chapter 2 Utility of Animal Models for Human Ovarian Tissue CryopreservationSuccess in cryopreservation of ovarian tissue (OT) in animal models has led to develop efficient cryo-technologies for human ovarian tissue. In this chapter, cryopreservation protocols developed for animal experiments are described.
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https://doi.org/10.1007/978-1-4939-6828-2Embryo cryopreservation; Embryo vitrification; Cryobiology; Human oocytes; ovarian and testicular tissue
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978-1-4939-8310-0Springer Science+Business Media LLC 2017
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