Titlebook: Cryopreservation and Freeze-Drying Protocols; Willem F. Wolkers,Harriëtte Oldenhof Book 2021Latest edition Springer Science+Business Media

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Mathematical Modeling of Protectant Transport in Tissuesul for design of preservation methods. Cell membrane transport modeling has been successfully used to guide design of preservation methods for isolated cells. For tissues, though, there are several mass transfer modeling challenges that arise from phenomena associated with cells being embedded in a

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High-Speed Video Cryomicroscopy for Measurement of Intracellular Ice Formation Kineticsing procedures for the cryopreservation of cells and tissues. Conventional cryomicroscopic assays, which rely on indirect evidence of intracellular freezing (e.g., opacity changes in the cell cytoplasm), can yield significant errors in the estimated kinetics. In contrast, the formation and growth of

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Use of Ice Recrystallization Inhibition Assays to Screen for Compounds That Inhibit Ice Recrystalliz these assays are the splat cooling assay (SCA) and sucrose sandwich assay (SSA). These two assays possess similarities; however, they vary in their sample size, cooling rate, and the solution used to dissolve the analyte. In this chapter, both assay methods are described in detail, and we perform a

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DSC Analysis of Thermophysical Properties for Biomaterials and Formulationsand protectant solutions involved. This chapter provides an introduction on the use of differential scanning calorimetry (DSC) to study thermophysical properties of biomaterials in protective solutions. It covers specific methods to study thermal events related to freezing and drying processes inclu

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Osmometric Measurements of Cryoprotective Agent Permeation into Tissuesis chapter details two inexpensive methods to measure cryoprotective agent permeation into tissues as functions of time. One of the methods to measure the CPA permeation is to permeate a series of tissue samples from a surrounding solution at a specified concentration of CPA, each sample for a diffe