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Titlebook: Cryopreservation and Freeze-Drying Protocols; Willem F. Wolkers,Harriëtte Oldenhof Book 2021Latest edition Springer Science+Business Media

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发表于 2025-3-21 19:10:56 | 显示全部楼层 |阅读模式
书目名称Cryopreservation and Freeze-Drying Protocols
编辑Willem F. Wolkers,Harriëtte Oldenhof
视频videohttp://file.papertrans.cn/241/240512/240512.mp4
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cryopreservation and Freeze-Drying Protocols; Willem F. Wolkers,Harriëtte Oldenhof Book 2021Latest edition Springer Science+Business Media
描述This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Thorough and authoritative, .Cryopreservation and Freeze-Drying Protocols, Fourth Edition. serves as an ideal guide for researchers and technical assist
出版日期Book 2021Latest edition
关键词Cryobiology; Vitrification; Cryostorage; Freeze-dried specimens; Human samples; Animal samples; Plant samp
版次4
doihttps://doi.org/10.1007/978-1-0716-0783-1
isbn_softcover978-1-0716-0785-5
isbn_ebook978-1-0716-0783-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
The information of publication is updating

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1064-3745 lls. .Thorough and authoritative, .Cryopreservation and Freeze-Drying Protocols, Fourth Edition. serves as an ideal guide for researchers and technical assist978-1-0716-0785-5978-1-0716-0783-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
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A Review on Stability of Caisson Breakwater,agents. Different mechanistic modes of action of cryoprotective and lyoprotective agents are described including minimizing ice formation, preferential exclusion, water replacement, and vitrification. Furthermore, it is discussed how protective agents can be introduced into cells avoiding damage due
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An Experiment in Writing that Flows,r models that can be used to describe transport phenomena occurring during loading of tissues with protective molecules for cryopreservation applications. Assumptions and simplifications that limit the applicability of each of these models are discussed.
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Wavelets Theory and Its Applicationsperature profile design, sample preparation, and configuration of the video acquisition parameters. Throughout this chapter, the protocols incorporate best practices that have been drawn from two decades of experience with high-speed video cryomicroscopy in our laboratory.
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Introduction to Numerical Methods time, which requires fewer tissue samples, is to measure the CPA efflux as a function of time. In the efflux method, a CPA-permeated tissue sample is placed in a surrounding solution, and solution samples are taken at different time points throughout the efflux to quantitate how much CPA has left t
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