Titlebook: Confocal Microscopy; Methods and Protocol Stephen W. Paddock Book 19991st edition Humana Press 1999

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Live Confocal Analysis with Fluorescently Labeled Proteins,diated phenotypes often can be accomplished only through live fluorescence analysis. Finally, live analysis has been used to confirm the existence of structures in the embryo that were previously contested as possible fixation artifacts (.).

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Book 19991st editionmens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.......

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is biological arena is the tracking of calcium levels in different subcellular regions. Our goal is to consider how fluorescent calcium indicators, in conjunction with confocal microscopy, can best be used to image subcellular calcium dynamics.

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Confocal Methods for ,,ltaneously. A confocal microscope equipped with a krypton/argon laser can simultaneously detect up to three different antigens. Using a confocal microscope it is also possible to collect a series of optical sections through a sample that allows observation of changes in distribution of the antigen in different focal planes of the tissue or cell.

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Book 19991st editionthe bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnic

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er scanning confocal microscope (LSCM) is somewhat better than that achieved in a conventional, wide-field light microscope (theoretical maximum resolution of 0.2 μm), but not as great as that in the transmission electron microscope (0.1 nm), it has bridged the gap between these two commonly used te

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structions can be made surpasses the limitations of conventional “wide-field” microscopic techniques where microtome sectioning is often required and cells must be viewed as flat, two-dimensional objects. Furthermore, the reduction in out-of-focus flare increases depth discrimination.