Titlebook: Confocal Microscopy; Methods and Protocol Stephen W. Paddock Book 19991st edition Humana Press 1999

Occlusion

dary antibodies and chromogenic substrates. The use of alkaline phosphatase substrates and their diffusible products limits the resolution of staining, particularly in thick tissues and deep within the embryo. Without additional probes, double-labeling and interpretation of results is also very difficult.

FLIT

accordance

hypnotic

抒情短诗

imaging cells deep within later stage embryos. Second, immunofluorescence and confocal detection allow the selective and simultaneous labeling with up to three different primary antisera. The following protocol contains instructions for the fixation, labeling, and detection of . embryos with one, two, and three different primary antisera.

做作

determine the genetic and epigenetic mechanisms that choreograph these events, it is often very useful to follow the morphogenetic behaviors of single cells and cell populations within the living zebrafish embryo.

寡头政治

实施生效

Imaging Gene Expression Using Antibody Probes, are physically labeled with dextran coupled to a fluorophore (.) or are labeled with fluorescent lipophilic dyes, such as diI (.). Multiple labeling experiments are then performed comparing the expression of these cell markers with endogenous protein(s) of interest.

integrated

有帮助

Analyzing Morphogenetic Cell Behaviors in Vitally Stained Zebrafish Embryos, determine the genetic and epigenetic mechanisms that choreograph these events, it is often very useful to follow the morphogenetic behaviors of single cells and cell populations within the living zebrafish embryo.