Titlebook: Confocal Microscopy; Methods and Protocol Joseph Brzostowski,Haewon Sohn Book 2021 This is a U.S. government work and not under copyright p

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ZEISS Airyscan: Optimizing Usage for Fast, Gentle, Super-Resolution Imaging,zed 32-channel Airyscan detector. By improving resolution twofold and signal-to-noise ratio eightfold relative to conventional confocal microscopes while retaining confocal functionality, the Airyscan microscope has become a very popular super-resolution imaging tool for cell biologists. In this cha

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Protein-Retention Expansion Microscopy (ExM): Scalable and Convenient Super-Resolution Microscopy,expansion is uniform across observable length scales, enabling imaging of structures previously too small to resolve. ExM is compatible with any microscope and does not require expensive materials or specialized software, offering effectively sub-diffraction-limited imaging capabilities to labs that

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Visualizing Key Signaling Components of Macropinocytosis and Phagocytosis Using Confocal Microscopyrise to an internal compartment called the macropinosomes or phagosome, respectively. . provides a powerful system to understand the molecular mechanism of these two fundamental cellular processes that impact human health and disease. Recent developments in fluorescence microscopy allow direct visua

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Imaging GPCR-Mediated Signal Events Leading to Chemotaxis and Phagocytosis, a well-established model to study both processes. Recent studies show that a G-protein-coupled receptor (fAR1) mediate a signaling network to control reorganization of the actin cytoskeleton leading both the directional cell movement and the engulfment of bacteria. Many live cell imaging methods ha

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High-Throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains,artments of choice using automated confocal microscopy. This procedure, which combines HTP genetics and microscopy, results in the acquisition of thousands of images that can be analyzed in a systematic and quantitative way to identify morphology defects in the tagged subcellular compartments. This

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Studying Neuronal Biology Using Spinning Disc Confocal Microscopy,es that directly or indirectly compromise cytoskeletal stability. While the large size and complexity of the neurons grown in culture poses certain challenges for imaging, live-cell imaging is an excellent approach to determine the morphological consequences of such mutants. This protocol details th

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