Titlebook: Concepts and Applications of Stem Cell Biology; A Guide for Students Gabriela Rodrigues,Bernard A. J. Roelen Textbook 2020 Springer Nature

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Preimplantation Development: From Germ Cells to Blastocyst, zygote after fusion. The zygote undergoes a series of cleavage divisions which will give rise to a morula stage embryo. At around this stage, the first lineage segregation event takes place leading to the formation of an outer trophectoderm layer and a pluripotent inner cell mass that will give ris

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Origins of Pluripotency: From Stem Cells to Germ Cells, The embryonic origins of stem cells will be discussed. The differences and similarities between naïve and primed embryonic stem cells will be described, and you will learn about the gene regulatory networks and signalling pathways that regulate each of them. This will relate to different culture co

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Human Induced Pluripotent Stem (hiPS) Cells: Generation and Applications,duced pluripotent stem (iPS) cells were first generated and briefly mentions a selection of historical findings which led to this groundbreaking discovery. Then, you learn how human iPS (hiPS) cells are generated from a practical point of view: what are the advantages and disadvantages of using cert

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Cellular Reprogramming and Aging,olved in the aging process, and how we can use those pathways to increase the efficiency of the reprogramming protocol. Furthermore, we will describe two protocols to establish primary cultures of a “young” cell line (mouse embryonic fibroblasts—MEFs) and an adult/old cell line (primary cultures of

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Axial Stem Cells and the Formation of the Vertebrate Body,e of tasks. The organism’s entire body plan is laid down in a series of intricate and interconnected events that comprise various levels of organization, from intracellular processes to vast morphogenetic tissue movements. This means that the embryo’s early symmetries must be gradually broken and th

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Skeletal Muscle Development: From Stem Cells to Body Movement,ystems of the adult organism. In this chapter, this concept is illustrated by the example of the development of one tissue, namely skeletal muscle. Skeletal muscles are a component of the musculo-skeletal system which also includes the bones, tendons, nerves and blood vessels. All muscle stem cells

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