Titlebook: Computational Methods in Synthetic Biology; Mario Andrea Marchisio Book 2021Latest edition Springer Science+Business Media, LLC, part of S

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Computational Methods for the Design of Recombinase Logic Circuits,nges. Detection and processing of multiple signals are crucial for many synthetic biology applications. A variety of logic circuits operating in living cells have been implemented. One particular class of logic circuits uses site-specific recombinases mediating specific DNA inversion or excision. Re

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Web-Based Base Editing Toolkits: BE-Designer and BE-Analyzer,nduce specific nucleotide changes in target DNA requires complex procedures and additional donor DNAs. Furthermore, CRISPR nuclease-mediated DNA cleavage at target sites frequently causes large deletions or genomic rearrangements. In contrast, base editors that consist of catalytically dead Cas9 (dC

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Synthetic Gene Circuit Analysis and Optimization,oblems are of central importance: (1) given the model of a biomolecular circuit, elucidate whether it is capable of a certain behavior/functionality; and (2) starting from a pre-defined required functionality and a library of biological parts, find the biomolecular circuit that, built as a combinati

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Construction of Protein Expression Network,hroughput technologies. Protein network is a powerful approach to describe the molecular mechanisms of the biological events through protein–protein interactions. Here, we describe the computational methods used to construct the protein network using expression data. We provide a list of available t

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