Titlebook: Computational Methods in Synthetic Biology; Mario Andrea Marchisio Book 2021Latest edition Springer Science+Business Media, LLC, part of S

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Pamela B. Adelman,Susan A. Vogelinked together in chains or networks called pathways or networks of pathways. In a classical functional analysis, the biomolecules found to play a role in the biological status under investigation are members of a group of pathways that are not necessarily interconnected. However, interconnectivity

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Computational Design of Multiplex Oligonucleotide-Based Assays, provides efficient multiplex oligonucleotide design including specificity and primer dimer checks. Its fully automated workflow involves important design steps that use specific parameters to produce high-quality oligonucleotides. This chapter describes how these steps are computationally implemented by oli2go.

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Mario Andrea MarchisioIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/232781.jpg

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https://doi.org/10.1007/978-1-4615-4385-5nteractions. Here, we describe the computational methods used to construct the protein network using expression data. We provide a list of available tools and databases that can be used in constructing the network.

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CRISPR-ERA: A Webserver for Guide RNA Design of Gene Editing and Regulation,s and build genome-wide sgRNA library using CRISPR-ERA, which is one of the state-of-the-art designer webserver tools for sgRNA design based on a set of sgRNA design rules summarized from published reports.