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Titlebook: Comparative Embryo Culture; Methods and Protocol Jason R. Herrick Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2

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发表于 2025-3-21 18:12:24 | 显示全部楼层 |阅读模式
书目名称Comparative Embryo Culture
副标题Methods and Protocol
编辑Jason R. Herrick
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Comparative Embryo Culture; Methods and Protocol Jason R. Herrick Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2
描述This detailed volume contains embryo culture techniques that should inspire embryologists to consider comparative studies. The species included in this volume represent a broad range of taxa, whose embryos have vastly different culture requirements and developmental characteristics.  Among the species described in this volume, significant differences exist in the rates of cell division, the timing of the maternal to embryonic transition, the relative lipid content of the cytoplasm, the sensitivity of the embryo to specific environmental ions, the preferred nutrients and associated metabolic pathways used by the embryo, the timing and mechanisms of early lineage specification, the presence or absence of embryonic diapause, and the time from fertilization to implantation. Written for the highly successful .Methods in Molecular Biology. series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Comparative Embryo Culture: Methods and Protocols. serves as an ideal aid for scientists seeking another sp
出版日期Book 2019
关键词Biomedical research; Blastocysts; Fertility; Embryonic development; Comparative studies; Cell culture
版次1
doihttps://doi.org/10.1007/978-1-4939-9566-0
isbn_softcover978-1-4939-9568-4
isbn_ebook978-1-4939-9566-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
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发表于 2025-3-21 20:43:29 | 显示全部楼层
Culture of the Mouse Preimplantation Embryo,sed on balanced salt solutions, containing the carbohydrates pyruvate, lactate, and glucose as the sole energy sources. Such media used a bicarbonate–carbon dioxide buffer system to maintain the desired pH, and were typically supplemented with serum albumin, but lacked free amino acids. In contrast
发表于 2025-3-22 01:51:57 | 显示全部楼层
In Vitro Culture of Rat Preimplantation Embryos,ed animal models to study human diseases and conditions. In addition to in vitro fertilization (IVF), the ability to grow IVF-derived or in vivo-collected zygotes to a desired preimplantation stage (zygote to blastocyst) entirely in vitro has a great importance for studies of developmental biology a
发表于 2025-3-22 07:46:05 | 显示全部楼层
Enabling Hamster Embryo Culture System: Development of Preimplantation Embryos,es being studied. While the mouse model has been versatile for studying in vitro development of early embryos, other rodent species are important to gain insights into comparative early embryogenesis. The golden hamster (.) offers unique advantages to study cellular and molecular regulation of gamet
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发表于 2025-3-22 14:38:34 | 显示全部楼层
In Vitro Maturation, Fertilization, and Culture of Pig Oocytes and Embryos,roduction agriculture. Methods of oocyte maturation, fertilization, and culture all play an extremely important role in how the embryo, fetus, and offspring will develop. In this chapter, we discuss the historical methods and recent advances that have been essential in promoting efficient and compet
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发表于 2025-3-23 00:49:41 | 显示全部楼层
Production and Culture of the Bovine Embryo,for in vitro maturation of oocytes, capacitation of sperm, fertilization, and development of the resultant embryos to the blastocyst stage. The protocol can be easily modified to use oocytes collected by ultrasound-guided follicular aspiration.
发表于 2025-3-23 03:53:29 | 显示全部楼层
In Vitro Embryo Production in Sheep,o revolutionize the productive efficiency of livestock species. The development of technology for sequencing of whole genomes and mass screening of gene regulation has widened our approach to genetic profiling and mapping, as well as furthering our understanding of underlying physiological mechanism
发表于 2025-3-23 07:45:46 | 显示全部楼层
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