Titlebook: Collagen; Methods and Protocol Irit Sagi,Nikolaos A. Afratis Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 E

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Juan Martín Dabezies,Paula Ballesteros-Ariasprotocols presented herein may be readily adapted to define interactomes of other collagen types, as well as to determine comparative interactomes of normal and disease-causing collagen variants using quantitative isotopic labeling (SILAC)- or isobaric mass tags (iTRAQ or TMT)-based mass spectrometr

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Felicia Pratto,Iris Žeželj,Marija Branković modified (e.g., acetylated) N termini. In this chapter, we provide a protocol for tandem mass tag (TMT)-TAILS analysis and further discuss specific considerations regarding N-terminome data interpretation using Proteome Discoverer™ software.

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Cloning and Mutagenesis Strategies for Large Collagenscine approaches. Using collagen VII as an example, we will here describe a strategy for rapid and simple modification of cDNAs encoding large collagens. The method is flexible and can be used for the creation of point mutations, small or large deletions, and insertion of DNA.

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A Biomimetic Model for Mineralization of Type-I Collagen Fibrilsor horse tendon is self-assembled into fibrils and mineralized by HAP in vitro. The mineralization process is monitored by cryoTEM in combination with two-dimensional (2D) and three-dimensional (3D) stochastic optical reconstruction microscopy (STORM), which enables in situ and high-resolution visualization of the process.

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Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopyinformation that ssNMR can provide and describe the procedures involved in a ssNMR structural study, with particular focus on using dynamic nuclear polarization to enhance sensitivity for detecting hydroxylysine residues by ssNMR.

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Book 2019t importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of co