Titlebook: Cohesin and Condensin; Methods and Protocol Kyoko Yokomori,Katsuhiko Shirahige Book 2017 Springer Science+Business Media New York 2017 cohe

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Book 2017and function of cohesin and the related factor condensin in vitro and in vivo across different model organisms, as well as in human cells. .Cohesin and Condensin: Methods and Protocols .is divided into three parts: Part I explores various in vitro and in vivo systems used to study the fundamental me

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Biochemical and Functional Assays of Human Cohesin-Releasing Factor Wapln from chromosomes during both interphase and mitosis. Here, we describe in vitro protein binding assays between Wapl and a cohesin subcomplex, and cellular assays in human cells that probe the functions of Wapl in cohesin release.

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A Protocol for Measuring Mitotic Chromosome Condensation Quantitatively in Fission Yeast Cellsscently labeled positions on the same chromosome. In combination with an automated computational analysis pipeline, this assay enables the study of various candidate proteins for their roles in regulating genome topology during cell divisions.

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A Sister Chromatid Cohesion Assay Using , Egg Extractsm chromatin is incubated with these extracts, it is first duplicated within an assembled nucleus, and is then transformed into mitotic chromosomes, in each of which sister chromatids are juxtaposed with each other in a cohesin-dependent manner. Here we describe our protocols for assembling duplicate

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An In Vitro Assay for Monitoring Topological DNA Entrapment by the Chromosomal Cohesin Complex. Cohesin is a large, ring-shaped protein complex and is thought to entrap DNA molecules inside of its ring. The unique DNA association is central to cohesin function and requires its ATPase and another heterodimer complex called the cohesin loader. Here we describe the biochemical reconstitution of